d) Methane 4) – Which of the following supplies all the carbon atoms that are needed for de novo synthesis of cholesterol? Start studying MCQ #2. d) Amino alcohols c) Adipolysis is inhibited. Taking aspirin has been shown to be cardioprotective in myocardial infarction. Cholesterol also serves as a precursor for the biosynthesis of steroid hormones, bile acid and vitamin D.Cholesterol is the principal sterol synthesized by all animals. For the production of 1 mole of cholesterol, 18 moles of Acetyl coA, 36 moles of ATP and 16 moles of NADPH are required. It is absent among prokaryotes (bacteria and archaea), although there are some exceptions, such as Mycoplasma, which require cholesterol for growth. The infant is diagnosed with medium chain acyl co A dehydrogenase deficiency. a) Progesterone Wang, 2000). How many carbons are present in HMG co-A? 15) A 45 -year-old female presents with pain in the right hypochondrium radiating towards shoulder and back. 6) Glycerol produced from hydrolysis of triglycerides in adipose tissue is a waste product, since it has to be in glycerol-3-P form and the enzyme for phosphorylation, glycerol kinase is absent in the adipose tissue. © 2011-2020 Sanfoundry. In molecular biology, hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). d) Ethylene A typical North American diet contains approximately 450mg of cholesterol per day (of which 55% is absorbed), while cholesterol synthesis on such a modest cholesterol diet is 11–13mg/kg/day. 21) A 20- year-old woman has a myocardial infarct and is given an Aspirin to chew. Sanfoundry Global Education & Learning Series – Biochemistry. Squalene 10 carbon intermediate 15 carbon intermediate d) 11 View Answer, 9. View Answer, 4. 10) Which of the following is a break down product of odd chain fatty acids? c) Non-steroid anti-inflammatory drug Which is the first intermediate in cholesterol synthesis? Disconnection: Synthetic steps for the disconnection shown: The key intermediates in cholesterol biosynthesis are HMG-CoA, mevalonic acid, isopentenyl pyrophosphate, and squalene. e) Pyruvate to acetyl co A conversion is inhibited. c) HMG co-A reductase NADPH provides the reducing equivalents. 1-d) Thyroid hormone synthesis 2-a) Phosphatidyl choline 3-c) Triacylglycerol 4-d) Cholesterol 5-a) Linolenic acid 6-a) Cholesterol 7-c) Cardiolipin 8- b) Lipoprotein lipase 9-c) Impairment of uptake of cholesterol by tissue 10­-d) Acyl CoA 2) A gall stone that blocked the upper part of the bile duct would cause an –, d) Decreased excretion of fats in the feces, e) Incomplete lipid digestion and absorption. View Answer, 10. A simplified schematic of the pathway that displaces the most important steps is shown in Figure 3. How many carbons are present in HMG co-A? d) TCA cycle is inhibited. 7) Which of the following enzymes is required for releasing the newly synthesized fatty acid from the fatty acid synthase complex? 20) A male infant is born with ambiguous genitalia (from lack of androgens such as testosterone) and several salt wasting (due to lack of aldosterone). Labels: Biochemistry mcq, biomolecules mcq, mcq on lipids, multiple choice on lipids, multiple choice questions on lipids, multiple choice questions on lipids Newer Post Older Post Home Classification of Lipids simplified in 8 minutes Testosterone is produced from which of the following compounds? Cholesterol synthesis takes place in the cytoplasm and in the endoplasmic reticulum (ER). Cholesterol is 27 carbon containing compound. Cholesterol is a precursor of Vit D and many steroid hormones like cortisol, estrogen, testosterone, etc. Two farnesyl-PP are condensed together: Supporting users have an ad free experience! View Answer, 8. Cholesterol Synthesis: Stage 3 Isoprenoids react with each other to form geranyl pyrophosphate. a) Mevalonate. Participate in the Sanfoundry Certification contest to get free Certificate of Merit. He is diagnosed with Respiratory distress syndrome due to deficiency of lung surfactant. Which of the following regulates the female reproductive cycle? b) Ethylene [1 kg] [1 g] [2 g] [2 kg] 7 people answered this MCQ question 1 g is the answer among 1 kg,1 g,2 g,2 kg for the mcq In adults, synthesis of cholesterol per day is about; Using in vitro and in vivo assays, we show that lanosterol, the first sterol intermediate in cholesterol synthesis, potently stimulates ubiquitination of reductase, whereas cholesterol has no effect at 10-fold higher concentrations. 1. Lipid metabolism is the synthesis and degradation of lipids in cells where fat are broken down for energy. In vertebrates, hepatic cells typically produce the greatest amounts. A comprehensive database of cholesterol quizzes online, test your knowledge with cholesterol quiz questions. 19) An infant is born prematurely at 28 weeks and has developed increasing difficulty in breathing. b) 9 These first two reactions, catalyzed by thiolase and HMG-CoA synthase, respectively, are reversible and do not commit the cell to the synthesis of cholesterol or other isoprenoid compounds. b) Fatty acid synthesis is impaired. d) Desmosterol. Which of (a)-(d) does not include a suitable first reagent? Oxidative Phosphorylation & Photophosphorylation, here is complete set of 1000+ Multiple Choice Questions and Answers, Prev - Biochemistry Questions and Answers – Biosynthesis of Membrane Phospholipids, Next - Biochemistry Questions and Answers – Overview of Nitrogen Metabolism, Biochemistry Questions and Answers – Biosynthesis of Membrane Phospholipids, Biochemistry Questions and Answers – Overview of Nitrogen Metabolism, Biochemistry Questions and Answers – Nitrogen Excretion and the Urea Cycle, Biochemistry Questions and Answers – Regulation of Oxidative Phosphorylation, Biochemistry Questions and Answers – ATP Synthesis – 2, Biochemistry Questions and Answers – Lipids as Signals, Cofactors, and Pigments, Best Reference Books – Chemistry of Lipids, Steroids and Hormones, Biochemistry – Advanced Questions and Answers, Biochemistry Questions and Answers – Analysis of Metabolic Control, Biochemistry Questions and Answers – Structural Lipids in Membranes, Biochemistry Questions and Answers – G Protein – Coupled Receptors and Second Messengers, Biochemistry Questions and Answers – Multivalent Scaffold Proteins and Membrane Rafts, Biochemistry Questions and Answers – Storage Lipids, Biochemistry Questions and Answers – Pentose Phosphate Pathway of Glucose Oxidation, Biochemistry Questions and Answers – Regulatory Enzymes, Biochemistry Questions and Answers – Protein Targeting and Degradation, Biochemistry Questions and Answers – Biosynthesis of Amino Acids, Biochemistry Questions and Answers – The Composition and Architecture of Membranes, Biochemistry Questions and Answers – Biosynthesis of Triacylglycerols, Biochemistry Questions and Answers – Biosynthesis of Fatty Acids and Eicosanoids. b) Testosterone Biosynthesis is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. 6. The reactions in this initial stage are the same as in ketogenesis (see slide 10.4.1).However, while ketogenesis occurs in the mitochondria, HMG-CoA destined for sterol synthesis … Which is the first intermediate in cholesterol synthesis? Multiple Choice Questions (MCQ) in Lipids, Fats & Wax with Detailed Answer Key. She is diagnosed with acute cholecystitis (inflammation of the gall bladder).The gall stones can obstruct the gall bladder, leading to an inadequate concentration of bile salts in the intestine. SOURCES OF CHOLESTEROL 12/14/13 Biochemistry for medics Cholesterol is derived from diet de novo synthesis and from the hydrolysis of cholesteryl esters. B) choline. Definition. A little more than half the cholesterol of the body arises by synthesis (about 700 mg/d), and the remainder is provided by the average diet. a) Thiolase Biochemistry Quizzes for CSIR NET Exam During reaction give off inorganic phosphate and make sure reaction only goes in one direction: Term. Cholesterol synthesis, also called cholesterologenesis, is a multistep enzymatic biosynthetic process that begins with acetyl-coenzyme A. View Answer, 7. His skin starts to turn blue from lack of oxygen(cyanosis). 11) Lipogenesis is increased when sucrose is fed instead of glucose because-, a) Sucrose is digested and absorbed rapidly, b) Sucrose metabolism causes release of insulin, c) Fructose, the hydrolytic product activates acetyl co A carboxylase, d) Fructose bypasses the phosphofructokinase control point in glycolysis and floods the pathway. e) Bile salts are synthesized from glycine and taurine. The phosphorylation reactions are required to solubilize the isoprenoid intermediates in the pathway. View Answer, 3. Join our social networks below and stay updated with latest contests, videos, internships and jobs! Which of the following is the major point of regulation on the pathway to cholesterol? c) 10 Cyclo-oxygenase is required for the production of which of these? Generally, the receptors for steroid hormones reside at ___________ e) 7- dehydro cholesterol b) Nuclear membrane Why are the intermediates of cholesterol synthesis pyrophosphates? 8) Which of the following is the key regulatory enzyme of fatty acid synthesis? C) lipoic acid. The essential intermediates in the pathway from acetate to cholesterol are _____ a) Acetic acid b) Ethylene Which of the following statements best describes the bile salts? The liver and intestine account for approximately 10% each of total synthesis in humans. The mevalonate pathway, also known as the isoprenoid pathway or HMG-CoA reductase pathway is an essential metabolic pathway present in eukaryotes, archaea, and some bacteria. Cholesterol synthesis starts with acetyl-CoA, which is used to synthesize hydroxymethylglutaryl-CoA (HMG-CoA). E) … 7-dehydrocholesterol is an immediate precursor to cholesterol that can be converted to vitamin D. Ergosterol is the cholesterol equivalent found in yeast. d) Plasma membrane Definition. 18) Which of the following statements best describes cholesterol ester transfer protein (CETP)? advertisement. Which of the following lipoproteins does not contain Apo B protein? Biological Oxidation and Electron transport chain, Gray Baby syndrome and the role of Glucuronic acid. a) Thiolase Which of the following is the integral membrane protein of smooth ER? b) HMG co-A synthase Tweet . Cholesterol’s synthesis from zymosterol requires an additional two NADPH to rearrange/remove double bonds. β-sitosterol is a major phytosterol, that is a sterol found in plants. 24,25-epoxycholesterol is an oxysterol produced in a shunt of the cholesterol … Save my name, email, and website in this browser for the next time I comment. b) HMG co-A synthase The essential intermediates in the pathway from acetate to cholesterol are ___________ a) Sterol nucleus with two alkyl chain attached to the ring D of cholesterol a) Acetic acid A membrane lipid is a compound, which belongs to a group of which form the double-layered surfaces of all cells. Synthesis of cholesterol begins with the transport of acetyl-CoA from within the mitochondria to the cytosol. d) Pyruvate kinase Which of the following is the phospholipid of primary importance in surfactant? 14)- Which of the following is not an intermediate of pathway of cholesterol synthesis ? The biosynthesis of cholesterol may be divided into five steps: (1) Synthesis of mevalonate from acetyl-CoA. All the carbon atoms in the cholesterol is provided by acetate. - Cholesterol can be synthesized by skin, intestine and kidney - major amounts of cholesterol but the main site of production is the liver What is the first intermediate for cholesterol synthesis? c) Isoprene units Lathosterol and desmosterol are intermediates that are commonly measured in blood samples. 14)- Which of the following is not an intermediate of pathway of cholesterol synthesis ? In cholesterol synthesis, six molecules of isopentenyl phosphate condense to form squalene, which is converted to lanosterol and then to cholesterol by a series of enzymatic reactions. c) Estrogens a) Mevalonate The key enzyme in the regulation of fatty acid synthesis is A. acetyl CoA carboxylase B. AMP activated proteinkinase C. protein phosphatase D. none of these Answer & Explanation Answer: Option A 2. Your email address will not be published. 1. b) Isoprene all isoprenoids, including cholesterol, steroids, prenylated proteins, heine A, dolichol, ubiquinone, carotenoids, retinoids, chlorophyll and natural rubber (K.C. Which of the following molecular complexes in the blood carries the palmitate residue from the lumen of the gut to the surface of the gut epithelial cell? All the carbons of cholesterol are derived from acetyl-CoA. a) 8 13) A 4 month -old infant presents with a seizure. Glycerol -3-P can still be produced in the adipose tissue through interconversion of one of the glycolytic intermediate. HMG-CoA is an intermediate in both cholesterol synthesis … Learn vocabulary, terms, and more with flashcards, games, and other study tools. The infant is found to have a profoundly low serum glucose and ketone body level. The cholesterol biosynthesis pathway involves enzymes that are in the cytoplasm, microsomes (ER), and peroxisomes. a) Nucleoplasm Prednisolone and prednisone are chemically ___________ c) 5 Steroids have ___________ a) It converts cholesterol from LDL to cholesterol esters, b) It is the major protein of Chylomicrons, c) It exchanges cholesterol esters from HDL for other lipids, e) It transfers cholesteryl esters from peripheral tissues to HDL. Condensation with another isopentyl-PP yields farnesyl pyrophosphate. The biosynthesis pathway of cholesterol is endergonic which require ATP. This set of Biochemistry Question Bank focuses on “Biosynthesis of Cholesterol, Steroids and Isoprenoids”. View Answer, 5. View Answer, 2. 17) In Abetalipoproteinemia (a rare disease), lipoproteins containing apo B are not formed and lipid droplets accumulate in the intestine and liver. To practice Biochemistry Question Bank, here is complete set of 1000+ Multiple Choice Questions and Answers. When Stearic acid is oxidized, how many Acetyl co A are produced? D) malate. c) HMG co-A reductase Designed by Abhishek Kashyap | Copyright Reserved ©2020 Namrata Chhabra, Lipid Metabolism- Multiple choice questions- set-1. d) Pyruvate kinase Arachidonate has 20 carbon atoms with A. What is the biochemical basis of infant’s symptoms? How is Squalene produced? 3)  A molecule of Palmitic acid attached to carbon 1 of glycerol moiety of a triacylglycerol is ingested and digested. a) Steroids 5) – Which of the following is not needed for the de novo synthesis of fatty acids? c) Cytoplasm Explanation: The first intermediate in cholesterol synthesis is mevalonate. Analysis of intermediate metabolites in cholesterol biosynthesis suggests that the first oxidative step in cholesterol biosynthesis catalyzed by squalene monooxygenase (SQLE), an important regulator downstream HMG-CoA reductase in cholesterol synthesis, is reduced by degradation of SQLE upon elevation of cellular plasmalogen level. Data suggested that lanosterol, the first intermediate in the pathway that is cyclic instead of linear, was the key regulator, but the researchers knew that a slightly modified version of lanosterol might be … The major regulatory enzyme is HMG-CoA reductase. Take up the gi lipid metab & membrane lipids MCQ's below to see how well you understood the course. Biochemistry Lipids Exam, Quiz on Lipids. The rate limiting step in cholesterol synthesis occurs at the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reducatase, HMGR, catalyzed step. 1) Which of the following is the best marker for the diagnosis of Acute pancreatitis? c) Lanosterol. The third reaction is the committed step: the reduction of HMGCoA to mevalonate, for which two molecules of NADPH each donate two electrons. View Answer. (2) Formation of isoprenoid units from mevalonate by loss of CO2. The resulting supernatant, a crude organelle fraction, was layered onto the sucrose density gradient (20–70%, wt/vol), and centrifuged at 113,000 × gfor 19 h at 4 C in a s… Squalene synthase is a 47-kDa protein of the endoplasmic reticulum and catalyzes the first committed step in cholesterol synthesis. Metabolic Biochemistry > MCQ Lipid Synthesis > Flashcards Flashcards in MCQ Lipid Synthesis Deck (14) Loading flashcards ... A thioester derivative of D-β-hydroxybutyrate is an intermediate in the synthetic path, ... Cholesterol is synthesized from: A) acetyl-CoA. His mother reports that her infant has been irritable and lethargic over the past several days. d) Release of Arachidonic acid from membrane Phospholipids, e) Hydroperoxy eicosa tetra enoic acid (HPETEs) from Arachidonic acid, Your email address will not be published. To collect SGs, the insulin peak was identified in the fractionated samples (16). d) Sterol nucleus but lack the alkyl chain attached to the ring D of cholesterol Which of the following glycolytic intermediates can be used for the synthesis of glycerol-3-P? a) 2 b) 3 c) 5 d) 6 View Answer. 16) Which of the following apo proteins is an activator of LCAT? d) Glucocorticoids How many carbons are present in geranyl pyrophosphate? a) Squalene and Lanosterol are examples of primary bile salts, b) Bile salts are required for transportation of lipids from intestine to liver, c) 95% of the bile salts are excreted in the feces per day, d) They are required for digestion and absorption of lipids. a) 2 … b) 3 3 double bonds B. (4) Cyclization of squalene to give rise to the parent steroid, lanosterol. He is diagnosed with a 3-β-hydroxylase deficiency. a) Beta oxidation of fatty acid is impaired, e) Pyruvate to acetyl co A conversion is inhibited. 12) An obese, 40- year-old woman, who recently underwent gastric bypass surgery, presents with severe vomiting due to rapid intake of large quantities of food, which must be avoided after gastric bypass surgery. An important conversion of cholesterol that takes place is esterification with long-chain fatty acids (e.g., palmitic acid) in the endoplasmic reticulum, which blocks cholesterol’s polar head group and, thus, its inclusion as a membrane fluidity buffer. Squalene synthase catalyzes the condensation of two molecules of farnesyl-PP with reduction by NADPH to make squalene. 9) The “microsomal system “elongates saturated and unsaturated fatty acyl-CoAs (from C10 upward) by two carbons, using-. Required fields are marked *. c) Sterol nucleus without CH3 between C ring and D ring of cholesterol b) Squalene. Briefly, cell homogenate was centrifuged at 3000 × g for 2 min. b) Sterol nucleus with two CH3 between C and D ring and A and B ring of cholesterol Acetyl CoA c) Squalene Our online cholesterol trivia quizzes can be adapted to suit your requirements for taking some of the top cholesterol quizzes. She had successfully lost 10 pounds over the last month after the surgery due to mobilization of fat stores to provide acetyl co A and energy. This reaction comprises the second step in the mevalonate-dependent isoprenoid biosynthesis pathway. Aspirin is a non-steroidal anti-inflammatory drug that inhibits cyclooxygenase enzyme (COX). b) Glycerol derivatives View Answer, 6. All Rights Reserved. (3) Condensation of six isoprenoid units to form squalene. d) 6 A disconnection of 1,4-diphenylbut-2-yne is given below followed by possible corresponding synthetic reactions. 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Networks below and stay updated with latest contests, videos, internships and jobs protein.: 1 following supplies all the carbon atoms that are commonly measured in blood samples blue from lack oxygen! Stearic acid is impaired, e ) Pyruvate to acetyl co a is... Her infant has been irritable and lethargic over the past several days of.. Cyclization of squalene to give rise to the parent steroid, lanosterol are required solubilize. Fats & Wax with Detailed Answer key broken down for energy learn vocabulary, terms and... Homogenate was centrifuged at 3000 × g for 2 min acetyl co a is! Electron transport chain, Gray Baby syndrome and the role of Glucuronic acid, microsomes ( ER,... Been shown which is the first intermediate in cholesterol synthesis mcq be cardioprotective in myocardial infarction cells where fat are broken down for.... Glucuronic acid in humans down product of odd chain fatty acids in lipids, &., cell homogenate was centrifuged at 3000 × g for 2 min cholesterol. 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